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Potassium restriction boosts vacuolar acidity and extends lifespan in yeast.

Authors
  • Sasikumar, Arjun N1
  • Killilea, David W2
  • Kennedy, Brian K3
  • Brem, Rachel B4
  • 1 Buck Institute for Research on Aging, Novato, CA, United States of America. , (United States)
  • 2 Nutrition & Metabolism Center and Elemental Analysis Facility, Children's Hospital Oakland Research Institute, Oakland, CA, United States of America. , (United States)
  • 3 Buck Institute for Research on Aging, Novato, CA, United States of America; Departments of Biochemistry and Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. , (Singapore)
  • 4 Buck Institute for Research on Aging, Novato, CA, United States of America; Department of Plant and Microbial Biology, UC Berkeley, Berkeley, CA, United States of America. Electronic address: [email protected] , (United States)
Type
Published Article
Journal
Experimental gerontology
Publication Date
Jun 01, 2019
Volume
120
Pages
101–106
Identifiers
DOI: 10.1016/j.exger.2019.02.001
PMID: 30742903
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Lysosome function is compromised during aging and in many disease states. Interventions that promote lysosomal activity and acidification are thus of prime interest as treatments for longevity and health. Intracellular pH can be controlled by the exchange of protons for inorganic ions, and in cells from microbes to man, when potassium is restricted in the growth medium, the cytoplasm becomes acidified. Here we use a yeast model to show that potassium limited-cells exhibit hallmarks of increased acidity in the vacuole, the analog of the lysosome, and live long by a mechanism that requires the vacuolar machinery. The emerging picture is one in which potassium restriction shores up vacuolar acidity and function, conferring health benefits early in life and extending viability into old age. Against the backdrop of well-studied protein and carbohydrate restrictions that extend lifespan and healthspan, our work establishes a novel pro-longevity paradigm of inorganic nutrient limitation. Copyright © 2019 Elsevier Inc. All rights reserved.

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