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Post-transcriptional gene silencing is involved in resistance of transgenic papayas to papaya ringspot virus

Authors
  • Ruanjan, P.1
  • Kertbundit, S.2
  • Juříček, M.3
  • 1 Mahidol University, Institute of Molecular Biology and Genetics, Salaya Campus, Nakhon Pathom, 73170, Thailand , Nakhon Pathom (Thailand)
  • 2 Mahidol University, Department of Plant Science, Faculty of Science, Rama VI Road, Payathai, Bangkok, 10400, Thailand , Payathai, Bangkok (Thailand)
  • 3 Academy of Sciences of the Czech Republic, Institute of Experimental Botany, Na Pernikářce 15, Prague 6, CZ-16000, Czech Republic , Prague 6 (Czechia)
Type
Published Article
Journal
Biologia Plantarum
Publisher
Kluwer Academic Publishers-Consultants Bureau
Publication Date
Sep 01, 2007
Volume
51
Issue
3
Pages
517–520
Identifiers
DOI: 10.1007/s10535-007-0110-0
Source
Springer Nature
Keywords
License
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Abstract

Transgenic papayas carrying the papaya ringspot virus coat protein gene were inoculated with Papaya ringspot virus. Infection was monitored by evaluating symptoms and by reverse transcription polymerase chain reaction (RT-PCR). Among eight tested transgenic lines, clone G2 was found highly resistant to virus infection during 3 years of testing. Further analysis of this clone revealed complex multicopy transgene insertion with aberrant copies. The suspected post transcriptional gene silencing was confirmed by siRNA detection. While the R0 generation of G2 transgenic papaya was found to be fully resistant to the infection, Papaya ringspot virus was able to break this resistance in subsequent generations by suppressing post-transcriptional gene silencing (PTGS).

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