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Possible cytokine biomarkers in pediatric acute appendicitis

  • Stankovic, Nikola1, 2
  • Surbatovic, Maja3, 4
  • Stanojevic, Ivan3, 4
  • Simić, Radoje1, 2
  • Djuricic, Slavisa1, 5
  • Milickovic, Maja1, 2
  • Grujic, Blagoje1, 2
  • Savic, Djordje1, 2
  • Marinovic, Vesna Milojkovic1, 2
  • Stankovic, Miona
  • Vojvodic, Danilo3, 4
  • 1 Mother And Child Health Care Institute of Serbia, Radoja Dakica 6, Belgrade, 11000, Serbia , Belgrade (Serbia)
  • 2 University of Belgrade, Faculty of Medicine, Belgrade, Serbia , Belgrade (Serbia)
  • 3 Military Medical Academy, Belgrade, Serbia , Belgrade (Serbia)
  • 4 University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia , Belgrade (Serbia)
  • 5 Banjaluka University School of Medicine, Banjaluka, Bosnia and Herzegovina , Banjaluka (Bosnia & Herzegovina)
Published Article
Italian Journal of Pediatrics
Springer (Biomed Central Ltd.)
Publication Date
Oct 15, 2019
DOI: 10.1186/s13052-019-0726-7
Springer Nature


BackgroundDiagnosis of acute appendicitis (AA) and decisions about its treatment remain among the most common dilemmas of pediatric surgical teams. Monitoring of immune response may be of importance for this purpose. Our aim was to measure and analyze serum and peritoneal fluid cytokines, in children who had undergone surgery for suspected AA.MethodsProspective investigation of serum and peritoneal fluid cytokine values was performed in 127 consecutive patients. According to the pathohistological findings, patients were divided into three groups: normal/early, uncomplicated and complicated AA. Determination of cytokine concentrations for 20 different cytokines was done using a commercial flow cytometry kit: Human Inflammation 20 plex BMS 819.ResultsStatistically significant differences in serum cytokine values between pathohistological groups were found for IP-10, MIP-1α and IL-10. Preoperative cut-off values of IP-10, MIP-1α and IL-10 between groups were obtained using ROC curve analysis. Positive correlations between serum and peritoneal concentrations were recorded for most of the analyzed cytokines.ConclusionIP-10, MIP-1α and IL-10 showed potential in assessment of AA in children. Confirmatory studies with a larger number of patients are required to prove reliability of these biomarkers.

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