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A positive feedback loop of heparanase/syndecan1/nerve growth factor regulates cancer pain progression

Authors
  • Su, Xiaohu
  • Wang, Bingwu
  • Zhou, Zhaoyun
  • Li, Zixian
  • Tong, Song
  • Chen, Simin
  • Zhang, Nan
  • Liu, Su
  • Zhang, Maoyin
Type
Published Article
Journal
The Korean Journal of Pain
Publisher
The Korean Pain Society
Publication Date
Jan 01, 2023
Volume
36
Issue
1
Pages
60–71
Identifiers
DOI: 10.3344/kjp.22277
PMID: 36536517
PMCID: PMC9812689
Source
PubMed Central
Keywords
Disciplines
  • Experimental Research Articles
License
Unknown

Abstract

Background The purpose of this research was to assess the role of heparanase (HPSE)/syndecan1 (SDC1)/nerve growth factor (NGF) on cancer pain from melanoma. Methods The influence of HPSE on the biological function of melanoma cells and cancer pain in a mouse model was evaluated. Immunohistochemical staining was used to analyze HPSE and SDC1. HPSE, NGF, and SDC1 were detected using western blot. Inflammatory factors were detected using ELISA assay. Results HPSE promoted melanoma cell viability, proliferation, migration, invasion, and tumor growth, as well as cancer pain, while SST0001 treatment reversed the promoting effect of HPSE. HPSE up-regulated NGF, and NGF feedback promoted HPSE. High expression of NGF reversed the inhibitory effect of HPSE down-regulation on melanoma cell phenotype deterioration, including cell viability, proliferation, migration, and invasion. SST0001 down-regulated SDC1 expression. SDC1 reversed the inhibitory effect of SST0001 on cancer pain. Conclusions The results showed that HPSE promoted melanoma development and cancer pain by interacting with NGF/SDC1. It provides new insights to better understand the role of HPSE in melanoma and also provides a new direction for cancer pain treatment.

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