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Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols

Authors
  • Volpato, Fabiana1, 2, 1
  • Lima-Morales, Daiana1, 1
  • Wink, Priscila Lamb1, 1, 3
  • Willig, Julia1, 4
  • de-Paris, Fernanda1
  • Ashton-Prolla, Patricia3, 5
  • Barth, Afonso Luís1, 2, 1, 3
  • 1 Hospital de Clínicas de Porto Alegre (HCPA),
  • 2 Universidade Federal do Rio Grande do Sul (UFRGS),
  • 3 UFRGS,
  • 4 Programa de Residência Multiprofissional do Hospital de Clínicas de Porto Alegre (HCPA),
  • 5 HCPA,
Type
Published Article
Journal
European Journal of Clinical Microbiology & Infectious Diseases
Publisher
Springer-Verlag
Publication Date
Oct 16, 2020
Pages
1–4
Identifiers
DOI: 10.1007/s10096-020-04071-8
PMID: 33063233
PMCID: PMC7561432
Source
PubMed Central
Keywords
License
Unknown

Abstract

This study has aimed to evaluate the use pool of samples as a strategy to optimize the diagnostic of SARS-CoV-2 by RT-qPCR. A total of 220 naso/orofaryngeal swab samples were collected and tested using two different protocols of sample pooling. Results from protocol A were identical with the individual results. However, for results from protocol B, reduced agreement (91%) was observed in relation to individual testing. Inconsistencies observed were related to RT-qPCR results with higher cycle thresholds. These results suggest that pooling of samples before RNA extraction is preferable in terms of diagnostic for SARS-CoV-2.

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