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Polysaccharide binding potential of the human A2 or A18 kappa light chain homologues.

Authors
Type
Published Article
Journal
Infection and immunity
Publication Date
Volume
67
Issue
2
Pages
994–997
Identifiers
PMID: 9916123
Source
Medline
License
Unknown

Abstract

Antibodies having light (L) chains encoded by the kappaII-A2 variable region gene segment predominate in the human response to the Haemophilus influenzae type b polysaccharide (Hib PS). To determine whether the closely related homologue of the A2 gene, the kappaII-A18 gene, has the potential to contribute to the repertoire, we examined Hib PS binding to a series of recombinant Fab fragments having either A2 or A18 L chains isolated from a Hib PS-vaccinated adult. The ability to bind Hib PS resided exclusively with those Fab fragments having A2 and containing an insertional arginine at the variable-joining junction. Thus, despite the sequence similarity between A2 and A18, only A2 contributes to the canonical Hib PS paratope.

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