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Polymer Conjugation to Enhance Cellulase Activity and Preserve Thermal and Functional Stability.

Authors
  • Wright, Thaiesha A1
  • Lucius Dougherty, Melissa1
  • Schmitz, Benjamin1
  • Burridge, Kevin M1
  • Makaroff, Katherine1
  • Stewart, Jamie M1
  • Fischesser, Henry D1
  • Shepherd, Jerry T1
  • Berberich, Jason A2
  • Konkolewicz, Dominik1
  • Page, Richard C1
  • 1 Department of Chemistry and Biochemistry, 651 East High Street, Miami University , Oxford, Ohio 45056 United States. , (United States)
  • 2 Department of Chemical, Paper, and Biomedical Engineering, 650 East High Street, Miami University , Oxford, Ohio 45056 United States. , (United States)
Type
Published Article
Journal
Bioconjugate Chemistry
Publisher
American Chemical Society
Publication Date
Oct 18, 2017
Volume
28
Issue
10
Pages
2638–2645
Identifiers
DOI: 10.1021/acs.bioconjchem.7b00518
PMID: 28934551
Source
Medline
License
Unknown

Abstract

A thermophilic cellulase, FnCel5a, from Fervidobacterium nodosum was conjugated with various functional polymers including cationic, anionic, and strongly and weakly hydrogen bonding polymers. The activity of FnCel5a toward a high-molecular-weight carboxymethyl cellulose substrate was enhanced by polymer conjugation. Activity enhancements of 50% or greater observed for acrylamide and mixed N,N-dimethyl acrylamide-2-(N,N-dimethylamino)ethyl methacrylate polymers, suggesting that the greatest enhancements were caused by polymers capable of noncovalent interactions with the substrate. The conjugates were found to have nearly identical thermodynamic stability to the native enzyme, as assessed by free energy (ΔG), enthalpy (ΔH), and entropy (TΔS) parameters extracted from differential scanning fluorimetry. Polymers tended to confer comparable tolerance to high concentrations of dimethylformamide, with longer polymers typically enabling higher activity relative to shorter polymers. The new FnCel5a conjugates represent an advance in the production of cellulases that maintain activity at high temperatures or in the presence of denaturing organic solvents.

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