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Poly-L-Lysine/Hyaluronan Nanocarriers As a Novel Nanosystem for Gene Delivery.

Authors
  • Souri, Masoumeh1, 2, 3
  • Bagherzadeh, Mohammad Aref4
  • Jahromi, Mirza Ali Mofazzal5, 6, 7
  • Mohammad-Beigi, Hossein8
  • Abdoli, Amir9, 10
  • Mir, Hamed11
  • Roustazadeh, Abazar11
  • Pirestani, Majid12
  • Zangabad, Parham Sahandi3
  • Kiani, Jafar13, 14
  • Bakhshayesh, Amirmahmoud15
  • Jahani, Mehdi15, 16
  • Joghataei, Mohammad Taghi1, 17
  • Karimi, Mahdi1, 2, 3
  • 1 Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. , (Iran)
  • 2 Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. , (Iran)
  • 3 Advances Nanobiotechnology and Nanomedicine Research Group (ANNRG), Iran University of Medical Sciences, Tehran, Iran. , (Iran)
  • 4 Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran. , (Iran)
  • 5 Research Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran. , (Iran)
  • 6 Department of Immunology, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran. , (Iran)
  • 7 Department of Advanced Medical Sciences & Technologies, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran. , (Iran)
  • 8 Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads, DK-2800 Kgs, Lyngby, Denmark. , (Denmark)
  • 9 Department of Parasitology, and Mycology, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran. , (Iran)
  • 10 Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran. , (Iran)
  • 11 Department of Biochemistry and Nutrition, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran. , (Iran)
  • 12 Parasitology and Entomology Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. , (Iran)
  • 13 Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. , (Iran)
  • 14 Oncopathology Research Center, Iran University of Medical Sciences, Tehran, Iran. , (Iran)
  • 15 Department of Physics, Sharif University of Technology, Tehran, Iran. , (Iran)
  • 16 Department of Chemistry, Sharif University of Technology, Tehran, Iran. , (Iran)
  • 17 Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. , (Iran)
Type
Published Article
Journal
Journal of microscopy
Publication Date
Apr 20, 2022
Identifiers
DOI: 10.1111/jmi.13107
PMID: 35443072
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The present research comes up with a novel DNA-loaded poly-l-lysine (PLL) / hyaluronan (HA) nanocarrier (DNA-loaded PLL/HA NCs) for gene delivery applications, as a promising candidate for gene delivery into diverse cells. A straightforward approach was employed to prepare such a nanosystem through masking DNA-loaded PLL molecules by HA. Fourier-transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), field emission-scanning electron microscopy (FE-SEM), and transmission electron microscopy (TEM) were used to analyze the interaction of the molecules as well as the physicochemical properties of the NCs. The NCs showed a negative charge of -24 ± 3 mV, with an average size of 138 ± 6 nm, in a ellipsoid-shape with smooth surfaces. The DNA loading efficiency (LE) measured by DNA absorbance was around 95 %. The MTT assay showed that the developed NCs are non-toxic to the cells. Furthermore,the uptake of the DNA-loaded PLL/HA NCs by the human embryonic kidney (HEK)-293T cells was evaluated by a flow cytometry method, and demonstrated high potential cellular uptake over 90% for transferring the gene to HEK-293T cells at the optimized conditions. Therefore, the DNA-loaded PLL/HA NCs are the potent strategy for developing nanosystems for gene delivery applications. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

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