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Polyethylene glycol interferes with protein molecular weight determinations by gel filtration.

Authors
Type
Published Article
Journal
Analytical biochemistry
Publication Date
Volume
138
Issue
1
Pages
137–140
Identifiers
PMID: 6731837
Source
Medline

Abstract

Gel filtration studies on Sephadex G-75 demonstrate markedly increased elution volumes for proteins chromatographed with polyethylene glycol. As little as 5% (w/v) polyethylene glycol in the applied protein sample can reduce apparent molecular weight estimates by gel filtration as much as 55%. Furthermore, gel filtration columns equilibrated with polyethylene glycol are not size-separating columns. Consequently, caution must be exercised when performing and interpreting gel filtration studies of proteins previously treated or precipitated with polyethylene glycol.

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