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[Polyclonal antibodies against human cell-surface antigen CD34].

Authors
Type
Published Article
Journal
T︠S︡itologii︠a︡ i genetika
Publication Date
Volume
45
Issue
3
Pages
3–14
Identifiers
PMID: 21774397
Source
Medline
License
Unknown

Abstract

An efficient and inexpensive laboratory approach for the generation and the purification of polyclonal antibodies to human antigen CD34 was developed. It was shown that cloned refolded and purified from Escherichia coli recombinant extracellular fragment of CD34 antigen retained immunogenic determinants of cell-surface expressed CD34. Immunization of mice with unglycosylated truncated recombinant protein elicit polyclonal antibodies specific for the native human antigen CD34. The antibodies generated are applicable for phenotyping of CD34+ cells using immunocytochemistry and flow cytometry assays.

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