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Polybromo protein BAF180 functions in mammalian cardiac chamber maturation.

Authors
  • Wang, Zhong
  • Zhai, Weiguo
  • Richardson, James A
  • Olson, Eric N
  • Meneses, Juanito J
  • Firpo, Meri T
  • Kang, Chulho
  • Skarnes, William C
  • Tjian, Robert
Type
Published Article
Journal
Genes & Development
Publisher
Cold Spring Harbor Laboratory
Publication Date
Dec 15, 2004
Volume
18
Issue
24
Pages
3106–3116
Identifiers
PMID: 15601824
Source
Medline
License
Unknown

Abstract

BAF and PBAF are two related mammalian chromatin remodeling complexes essential for gene expression and development. PBAF, but not BAF, is able to potentiate transcriptional activation in vitro mediated by nuclear receptors, such as RXRalpha, VDR, and PPARgamma. Here we show that the ablation of PBAF-specific subunit BAF180 in mouse embryos results in severe hypoplastic ventricle development and trophoblast placental defects, similar to those found in mice lacking RXRalpha and PPARgamma. Embryonic aggregation analyses reveal that in contrast to PPARgamma-deficient mice, the heart defects are likely a direct result of BAF180 ablation, rather than an indirect consequence of trophoblast placental defects. We identified potential target genes for BAF180 in heart development, such as S100A13 as well as retinoic acid (RA)-induced targets RARbeta2 and CRABPII. Importantly, BAF180 is recruited to the promoter of these target genes and BAF180 deficiency affects the RA response for CRABPII and RARbeta2. These studies reveal unique functions of PBAF in cardiac chamber maturation.

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