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Point-of-care HCV RNA testing in the setting of DAA therapy: HCV-FiS (HEpatitis C Virus Fingerstick Study).

Authors
  • Calvaruso, Vincenza1
  • Bronte, Fabrizio1
  • Ferraro, Donatella2
  • Reina, Giada1
  • Conte, Elisabetta1
  • Rini, Francesca1
  • Magro, Bianca1
  • Petta, Salvatore1
  • Di Marco, Vito1
  • Craxì, Antonio1
  • 1 Sezione di Gastroenterologia & Epatologia, PROMISE, University of Palermo, Palermo, Italy. , (Italy)
  • 2 Sezione di Virologia PROMISE, University of Palermo, Palermo, Italy. , (Italy)
Type
Published Article
Journal
Liver international : official journal of the International Association for the Study of the Liver
Publication Date
Dec 01, 2019
Volume
39
Issue
12
Pages
2240–2243
Identifiers
DOI: 10.1111/liv.14242
PMID: 31502755
Source
Medline
Language
English
License
Unknown

Abstract

HCV-RNA assessment during therapy with Direct-Acting Antiviral (DAA) regimens still relies on assays requiring blood collection and transport to a specialised laboratory, which may compromise linkage to care. GeneXpert-HCV Viral Load (GXHVL) (Cepheid) is a plasma-based assay used at point of care (POC) with a sensitivity of ≤10 IU/mL, and, results available within 2 hours. Fifty-nine consecutive HCV-patients ready for DAAs treatment were enrolled. HCV-RNA was simultaneously tested using Roche TaqMan RT-PCR (venous blood sample) and GXHVL (capillary blood collected by fingerstick), at baseline (BL), week 4 (W4) of therapy, end of therapy (EOT) and week 12 of follow-up (W12FU). Both assays demonstrated undetectable HCV-RNA in all patients at EOT and identified the single case of HCV-relapse at W12FU. GXHVL used as a point-of-care assay in the outpatient setting provides results fully comparable to the laboratory-based test. Its excellent performance and ease of use suggest its adoption in non-specialist settings where simplicity of care is paramount to implement HCV eradication campaigns. © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

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