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A point mutation to the long terminal repeat of bovine leukemia virus related to viral productivity and transmissibility.

Authors
  • Murakami, Hironobu1
  • Todaka, Haruna2
  • Uchiyama, Jumpei3
  • Sato, Reiichiro4
  • Sogawa, Kazuyuki5
  • Sakaguchi, Masahiro3
  • Tsukamoto, Kenji2
  • 1 Laboratory of Animal Health Ⅱ, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, 252-5201, Japan. Electronic address: [email protected] , (Japan)
  • 2 Laboratory of Animal Health Ⅱ, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, 252-5201, Japan. , (Japan)
  • 3 Laboratory of Veterinary Microbiology Ⅰ, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, 252-5201, Japan. , (Japan)
  • 4 Laboratory of Clinical Veterinary Medicine for Large Animal, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, 252-5201, Japan. , (Japan)
  • 5 Laboratory of Biochemistry, School of Life and Environmental Science, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, 252-5201, Japan. , (Japan)
Type
Published Article
Journal
Virology
Publisher
Elsevier
Publication Date
Aug 17, 2019
Volume
537
Pages
45–52
Identifiers
DOI: 10.1016/j.virol.2019.08.015
PMID: 31445323
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

It is important to establish the molecular basis of the high transmissibility of bovine leukemia virus (BLV) to develop new methods of preventing viral transmission. Hence, the aim of this study was to determine whether some strains had transmission advantages. First, we determined the whole BLV genome sequences of all 34 BLV-infected cows from one farm. Phylogenetic analysis divided strains into 26 major and 8 minor strains. The major strains dominantly spread independent of host factor, bovine leucocyte antigen. Further analysis, with molecular clones, associated transmissibility with viral productivity in vitro. In addition, the two groups could be classified by group-specific mutations. The reverse genetic approach demonstrated that a spontaneous mutation at nucleotide 175 of the BLV genome, which is located in the viral promoter region, could alter viral productivity by changing viral transactivation, suggesting that BLV transmissibility is affected by a spontaneous mutation associated with viral productivity. Copyright © 2019 Elsevier Inc. All rights reserved.

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