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PNAG-specific equine IgG1 mediates significantly greater opsonization and killing of Prescottella equi (formerly Rhodococcus equi) than does IgG4/7.

Authors
  • Rocha, Joana N1
  • Dangott, Lawrence J2
  • Mwangi, Waithaka3
  • Alaniz, Robert C4
  • Bordin, Angela I5
  • Cywes-Bentley, Colette6
  • Lawhon, Sara D7
  • Pillai, Suresh D8
  • Bray, Jocelyne M9
  • Pier, Gerald B10
  • Cohen, Noah D11
  • 1 College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 660 Raymond Stotzer Pkwy, College Station, TX 77843-4475, United States. Electronic address: [email protected] , (United States)
  • 2 Protein Chemistry Laboratory, Texas A&M University, 300 Olsen Blvd, College Station, TX 77843, United States. Electronic address: [email protected] , (United States)
  • 3 Department of Diagnostic Medicine/Pathobiology, Kansas State University, 1800 Denison Ave, Manhattan, KS 66506, United States. Electronic address: [email protected] , (United States)
  • 4 Department of Microbial Pathogenesis and Immunology, Texas A&M University Health and Science Center, 206 Olsen Blvd, College Station, TX 77845, United States. Electronic address: [email protected] , (United States)
  • 5 College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 660 Raymond Stotzer Pkwy, College Station, TX 77843-4475, United States. Electronic address: [email protected] , (United States)
  • 6 Harvard Medical School, Brigham & Women's Hospital, 181 Longwood Ave, Boston, MA 02115, United States. Electronic address: [email protected] , (United States)
  • 7 College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 660 Raymond Stotzer Pkwy, College Station, TX 77843-4475, United States. Electronic address: [email protected] , (United States)
  • 8 National Center for Electron Beam Research-IAEA Collaborative Centre for Electron Beam Technology, Texas A&M University, 400 Discovery Dr, College Station, TX 77845, United States. Electronic address: [email protected] , (United States)
  • 9 College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 660 Raymond Stotzer Pkwy, College Station, TX 77843-4475, United States. , (United States)
  • 10 Harvard Medical School, Brigham & Women's Hospital, 181 Longwood Ave, Boston, MA 02115, United States. Electronic address: [email protected] , (United States)
  • 11 College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 660 Raymond Stotzer Pkwy, College Station, TX 77843-4475, United States. Electronic address: [email protected] , (United States)
Type
Published Article
Journal
Vaccine
Publication Date
Feb 21, 2019
Volume
37
Issue
9
Pages
1142–1150
Identifiers
DOI: 10.1016/j.vaccine.2019.01.028
PMID: 30691984
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Prescottella equi (formerly Rhodococcus equi) is a facultative intracellular bacterial pathogen that causes severe pneumonia in foals 1-6 months of age, whereas adult horses are highly resistant to infection. We have shown that vaccinating pregnant mares against the conserved surface polysaccharide capsule, β-1 → 6-linked poly-N-acetyl glucosamine (PNAG), elicits opsonic killing antibody that transfers via colostrum to foals and protects them against experimental infection with virulent. R. equi. We hypothesized that equine IgG1 might be more important than IgG4/7 for mediating protection against R. equi infection in foals. To test this hypothesis, we compared complement component 1 (C1) deposition and polymorphonuclear cell-mediated opsonophagocytic killing (OPK) mediated by IgG1 or IgG4/7 enriched from either PNAG hyperimmune plasma (HIP) or standard plasma. Subclasses IgG1 and IgG4/7 from PNAG HIP and standard plasma were precipitated onto a diethylaminoethyl ion exchange column, then further enriched using a protein G Sepharose column. We determined C1 deposition by enzyme-linked immunosorbent assay (ELISA) and estimated OPK by quantitative microbiologic culture. Anti-PNAG IgG1 deposited significantly (P < 0.05) more C1 onto PNAG than did IgG4/7 from PNAG HIP or subclasses IgG1 and IgG4/7 from standard plasma. In addition, IgG1 from PNAG HIP mediated significantly (P < 0.05) greater OPK than IgG4/7 from PNAG HIP or IgG1 and IgG4/7 from standard plasma. Our findings indicate that anti-PNAG IgG1 is a correlate of protection against R. equi in foals, which has important implications for understanding the immunopathogenesis of R. equi pneumonia, and as a tool for assessing vaccine efficacy and effectiveness when challenge is not feasible. Copyright © 2019 Elsevier Ltd. All rights reserved.

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