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Plasmonic nanoholes in a multichannel microarray format for parallel kinetic assays and differential sensing.

Authors
  • Im, Hyungsoon
  • Lesuffleur, Antoine
  • Lindquist, Nathan C
  • Oh, Sang-Hyun
Type
Published Article
Journal
Analytical Chemistry
Publisher
American Chemical Society
Publication Date
Apr 15, 2009
Volume
81
Issue
8
Pages
2854–2859
Identifiers
DOI: 10.1021/ac802276x
PMID: 19284776
Source
Medline
License
Unknown

Abstract

We present nanohole arrays in a gold film integrated with a six-channel microfluidic chip for parallel measurements of molecular binding kinetics. Surface plasmon resonance effects in the nanohole arrays enable real-time, label-free measurements of molecular binding events in each channel, while adjacent negative reference channels can record measurement artifacts such as bulk solution index changes, temperature variations, or changing light absorption in the liquid. With the use of this platform, streptavidin-biotin specific binding kinetics are measured at various concentrations with negative controls. A high-density microarray of 252 biosensing pixels is also demonstrated with a packing density of 10(6) sensing elements/cm(2), which can potentially be coupled with a massively parallel array of microfluidic channels for protein microarray applications.

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