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Plasma Lipidomics Investigation of Hemodialysis Effects by Using Liquid Chromatography-Mass Spectrometry.

Authors
  • Wang, Lichao1, 2
  • Hu, Chunxiu2
  • Liu, Shuxin3
  • Chang, Ming3
  • Gao, Peng2, 4
  • Wang, Lili1
  • Pan, Zaifa1
  • Xu, Guowang2
  • 1 College of Chemical Engineering, Zhejiang University of Technology , Hangzhou 310014, China. , (China)
  • 2 Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China. , (China)
  • 3 Nephrology Department, Dalian Municipal Central Hospital , 826 Xinan Road, Dalian 116033, China. , (China)
  • 4 Clinical Laboratory, Dalian Sixth People's Hospital , 269 Lugang Huibai Road, Dalian 116031, China. , (China)
Type
Published Article
Journal
Journal of Proteome Research
Publisher
American Chemical Society
Publication Date
Jun 03, 2016
Volume
15
Issue
6
Pages
1986–1994
Identifiers
DOI: 10.1021/acs.jproteome.6b00170
PMID: 27151145
Source
Medline
Keywords
License
Unknown

Abstract

Chronic kidney disease (CKD) has been a global health problem that has a great possibility of being developed into uremia in the end. Hemodialysis (HD) is the most commonly used strategy for treating uremic patients; however, the patients still have a high risk of suffering various complications. It is well recognized that lipid disorder usually occurs in maintenance HD patients. To systemically study the effects of HD on lipid metabolism associated with uremia, we employed an ultraperformance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based lipidomics method. A total of 87 human plasma samples from patients with prehemodialysis (pre-HD)/posthemodialysis (post-HD) treatment and the healthy controls were enrolled in the study. As compared with pre-HD patients, many plasma lipids showed significant changes (p < 0.05) in patients receiving HD therapy. Specifically, sum of free fatty acids (FFA) as well as saturated FFA and eicosanoids and sums of lyso-phosphatidylinositols and lyso-phosphatidylethanolamines, FFA 16:1/FFA 16:0, and FFA 18:1/FFA 18:0 were obviously higher in the pre-HD group than in the controls while they were significantly lower in patients after HD. These results indicated that UPLC-Q-TOF/MS-based lipidomics is a promising approach to investigate lipid alterations in relation to uremia and it is helpful to understand complex complications involved in HD patients.

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