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Plasma adrenocorticotropic hormone concentration in horses decreases after freezing for 60 days.

Authors
  • Haffner, John C1, 2
  • Neal, Dwana L1, 2
  • Hoffman, Rhonda M1, 2
  • Grubbs, Steven T1, 2
  • 1 Middle Tennessee State University, Murfreesboro, TN (Haffner, Hoffman).
  • 2 Boehringer Ingelheim Vetmedica Inc., St. Joseph, MO (Neal, Grubbs).
Type
Published Article
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Publication Date
Nov 01, 2019
Volume
31
Issue
6
Pages
856–858
Identifiers
DOI: 10.1177/1040638719882374
PMID: 31646941
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

We investigated the stability of adrenocorticotropic hormone (ACTH) in plasma after freezing for different lengths of time. The plasma ACTH concentrations of 12 horses were measured on day 0 (baseline) and over time, after stimulation with thyrotropin-releasing hormone. Samples were stored at -80°C for 3, 7, 30, 60, and 90 d, or at -20°C for 3, 7, 30, and 60 d, or between ice packs at -20°C for 3 and 7 d prior to determination of ACTH concentration. ACTH concentrations were compared to baseline (non-frozen day 0 plasma) for each storage method using a mixed model with repeated measures in which each horse served as its own control and day was the repeated effect. Statistical significance was set at p ≤ 0.05, and 0.05 < p < 0.10 was considered a trend. Plasma ACTH frozen at -20°C or at -80°C resulted in degradation of ACTH compared to baseline samples at 60 and 90 d respectively. There was no degradation of ACTH after 7 d when stored between ice packs, or before 30 d at -20°C, or before 60 d at -80°C.

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