Se deficiency is widespread in agricultural soils; hence, agronomic Se biofortification is an important strategy to overcome its deficiency in humans and animals. In Finland, fertilizers have been amended with inorganic Se for over 20 years to reverse the negative effects of low Se content in feed and food. Plant species, climatic conditions, other nutrients and soil properties affect the efficiency of Se biofortification. The present two years’ study compared the ability of oilseed rape, wheat and forage grasses to uptake fertilizer Se applied as sodium selenate in a sub-boreal environment. The effect of foliar N application on Se uptake was tested in the second year. Se concentration was determined in plant parts and in soil samples taken at the end of growth season in both years as well as from another plot where Se fertilizer had been used for 20 years. Se fertilizer recovery in harvested wheat and oilseed rape was 1–16%, and in forage grasses was 52–64% in the first harvest and 15–19% in the second harvest. Foliar N application improved Se uptake only at the higher Se fertilizer level. The efficiency of biofortification depended on weather conditions, with forage grasses being the most reliable crop. Oilseed rape as a Se semi-accumulator had no advantage in Se biofortification in field conditions due to low translocation to seeds.