Affordable Access

deepdyve-link
Publisher Website

A Pitx2-MicroRNA Pathway Modulates Cell Proliferation in Myoblasts and Skeletal-Muscle Satellite Cells and Promotes Their Commitment to a Myogenic Cell Fate.

Authors
  • Lozano-Velasco, Estefanía
  • Vallejo, Daniel
  • Esteban, Francisco J
  • Doherty, Chris
  • Hernández-Torres, Francisco
  • Franco, Diego
  • Aránega, Amelia Eva
Type
Published Article
Journal
Molecular and Cellular Biology
Publisher
American Society for Microbiology
Publication Date
Sep 01, 2015
Volume
35
Issue
17
Pages
2892–2909
Identifiers
DOI: 10.1128/MCB.00536-15
PMID: 26055324
Source
Medline
License
Unknown

Abstract

The acquisition of a proliferating-cell status from a quiescent state as well as the shift between proliferation and differentiation are key developmental steps in skeletal-muscle stem cells (satellite cells) to provide proper muscle regeneration. However, how satellite cell proliferation is regulated is not fully understood. Here, we report that the c-isoform of the transcription factor Pitx2 increases cell proliferation in myoblasts by downregulating microRNA 15b (miR-15b), miR-23b, miR-106b, and miR-503. This Pitx2c-microRNA (miRNA) pathway also regulates cell proliferation in early-activated satellite cells, enhancing Myf5(+) satellite cells and thereby promoting their commitment to a myogenic cell fate. This study reveals unknown functions of several miRNAs in myoblast and satellite cell behavior and thus may have future applications in regenerative medicine.

Report this publication

Statistics

Seen <100 times