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Pituitary adenylate cyclase-activating polypeptide enhances Ca(2+)-dependent neurotransmitter release from PC12 cells and cultured cerebellar granule cells without affecting intracellular Ca(2+) mobilization.

Authors
Type
Published Article
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publication Date
Volume
286
Issue
3
Pages
646–651
Identifiers
PMID: 11511109
Source
Medline

Abstract

The effects of pituitary adenylate cyclase-activating polypeptide (PACAP), a neuropeptide isolated from mammalian hypothalamus, was investigated on neurotransmitter release from clonal rat pheochromocytoma PC12 cells and cultured rat cerebellar granule cells. We found that PACAP38 stimulates the neurotransmitter release from PC12 cells by two distinct mechanisms in different concentration ranges. In the lower concentration range (<1 nM), PACAP38 enhanced depolarization- and ionomycin-dependent dopamine release without mobilizing intracellular Ca(2+), while in the higher concentration range (>1 nM), PACAP38 induced profound Ca(2+) influx and concomitant dopamine release from PC12 cells. In cultured rat cerebellar granule cells, PACAP38 failed to increase intracellular Ca(2+); however, it enhanced depolarization-dependent glutamate release remarkably. These results indicate that PACAP38 enhances Ca(2+)-dependent neurotransmitter release by modulating step(s) subsequent to Ca(2+) entry.

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