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Pitfalls in the use of multicolour flow cytometry in haematology.

Authors
  • Johansson, Ulrika
  • Macey, Marion
Type
Published Article
Journal
Journal of Clinical Pathology
Publisher
BMJ
Publication Date
Jul 01, 2011
Volume
64
Issue
7
Pages
561–563
Identifiers
DOI: 10.1136/jcp.2010.085183
PMID: 21450754
Source
Medline
License
Unknown

Abstract

Multicolour flow cytometry in haematology has developed considerably in recent years. The ability to analyse eight or more colours of fluorescence on millions of cells in a matter of minutes has enabled the provision of rapid and reliable measures of minimal residual disease for clinicians. The use of multicolour analysis has also enabled more specific characterisation of presenting leukaemias and lymphomas. However, there has not been a concomitant increase in the knowledge and experience of the flow cytometrists to deal with certain problems associated with this more complex analysis.

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