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Pitfalls in cytokine analysis in inflammatory bowel disease.

Authors
Type
Published Article
Journal
Alimentary pharmacology & therapeutics
Publication Date
Volume
10 Suppl 2
Identifiers
PMID: 8899103
Source
Medline
License
Unknown

Abstract

Analysis of cytokine production and function is an indispensable tool to study Crohn's disease and ulcerative colitis. This analysis has generated a tremendous amount of data, but a clear interpretation of results has been hampered by limited attention paid to several patient- and sample-related pitfalls. These include: clinical parameters; the relative value of circulating vs. intestinal cytokine levels; the selection of tissue specimens and their processing method; the specific cellular source of each cytokine; the effect of cytokine inducers; and the technique utilized to obtain qualitative or quantitative data on cytokine protein or mRNA. Once all of these crucial variables are taken into proper perspective, a better understanding will emerge of the true role of cytokines in the pathogenesis of inflammatory bowel disease.

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