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Pirfenidone inhibits TGF-beta expression in malignant glioma cells.

Authors
  • Burghardt, Isabel
  • Tritschler, Felix
  • Opitz, Christiane A
  • Frank, Brigitte
  • Weller, Michael
  • Wick, Wolfgang
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Mar 09, 2007
Volume
354
Issue
2
Pages
542–547
Identifiers
PMID: 17234158
Source
Medline
License
Unknown

Abstract

Due to its immunosuppressive properties, the cytokine transforming growth factor (TGF)-beta has become a promising target in the experimental treatment of human malignant gliomas. Here, we report that the antifibrotic drug 5-methyl-1-phenyl-2-(1H)-pyridone (pirfenidone, PFD) elicits growth-inhibitory effects and reduces TGF-beta2 protein levels in human glioma cell lines. This reduction in TGF-beta2 is biologically relevant since PFD treatment reduces the growth inhibition of TGF-beta-sensitive CCL-64 cells mediated by conditioned media of glioma cells. The downregulation of TGF-beta is mediated at multiple levels. PFD leads to a reduction of TGF-beta2 mRNA levels and of the mature TGF-beta2 protein due to decreased expression and direct inhibition of the TGF-beta pro-protein convertase furin. In addition, PFD reduces the protein levels of the matrix metalloproteinase (MMP)-11, a TGF-beta target gene and furin substrate involved in carcinogenesis. These data define PFD or PFD-related agents as promising agents for human cancers associated with enhanced TGF-beta activity.

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