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Pilot-scale production of xylo-oligosaccharides and fermentable sugars from Miscanthus using steam explosion pretreatment

Authors
  • Bhatia, Rakesh1
  • Winters, Ana1
  • Bryant, David N.1
  • Bosch, Maurice1
  • Clifton-Brown, John1
  • Leak, David2
  • Gallagher, Joe1
  • 1 Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Plas Gogerddan, Aberystwyth SY23 3EE, UK
  • 2 Department of Biology & Biochemistry, University of Bath, Bath BA2 7AY, UK
Type
Published Article
Journal
Bioresource technology
Publication Date
Jan 01, 2020
Volume
296
Identifiers
DOI: 10.1016/j.biortech.2019.122285
PMID: 31715557
PMCID: PMC6920740
Source
PubMed Central
Keywords
Disciplines
  • Article
License
Unknown

Abstract

This study investigated pilot-scale production of xylo-oligosaccharides (XOS) and fermentable sugars from Miscanthus using steam explosion (SE) pretreatment. SE conditions (200 °C; 15 bar; 10 min) led to XOS yields up to 52 % (w/w of initial xylan) in the hydrolysate. Liquid chromatography-mass spectrometry demonstrated that the solubilised XOS contained bound acetyl- and hydroxycinnamate residues, physicochemical properties known for high prebiotic effects and anti-oxidant activity in nutraceutical foods. Enzymatic hydrolysis of XOS-rich hydrolysate with commercial endo -xylanases resulted in xylobiose yields of 380 to 500 g/kg of initial xylan in the biomass after only 4 h, equivalent to ~74 to 90 % conversion of XOS into xylobiose. Fermentable glucose yields from enzymatic hydrolysis of solid residues were 8 to 9-fold higher than for untreated material. In view of an integrated biorefinery, we demonstrate the potential for efficient utilisation of Miscanthus for the production of renewable sources, including biochemicals and biofuels.

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