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A piggybacking mechanism enables peroxisomal localization of the glyoxylate cycle enzyme Mdh2 in yeast.

Authors
  • Gabay-Maskit, Shiran1
  • Cruz-Zaragoza, Luis Daniel2
  • Shai, Nadav1
  • Eisenstein, Miriam1
  • Bibi, Chen1
  • Cohen, Nir1
  • Hansen, Tobias2
  • Yifrach, Eden1
  • Harpaz, Nofar1
  • Belostotsky, Ruth3
  • Schliebs, Wolfgang2
  • Schuldiner, Maya4
  • Erdmann, Ralf5
  • Zalckvar, Einat4
  • 1 Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel. , (Israel)
  • 2 Abteilung für Systembiochemie, Institut für Biochemie und Pathobiochemie, Medizinische Fakultät, Ruhr-Universität Bochum, Bochum D-44780, Germany. , (Germany)
  • 3 Division of Pediatric Nephrology, Shaare Zedek Medical Center, Jerusalem, Israel. , (Israel)
  • 4 Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel [email protected] [email protected] [email protected] , (Israel)
  • 5 Abteilung für Systembiochemie, Institut für Biochemie und Pathobiochemie, Medizinische Fakultät, Ruhr-Universität Bochum, Bochum D-44780, Germany [email protected] [email protected] [email protected] , (Germany)
Type
Published Article
Journal
Journal of Cell Science
Publisher
The Company of Biologists
Publication Date
Dec 17, 2020
Volume
133
Issue
24
Identifiers
DOI: 10.1242/jcs.244376
PMID: 33177075
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Eukaryotic cells have evolved organelles that allow the compartmentalization and regulation of metabolic processes. Knowledge of molecular mechanisms that allow temporal and spatial organization of enzymes within organelles is therefore crucial for understanding eukaryotic metabolism. Here, we show that the yeast malate dehydrogenase 2 (Mdh2) is dually localized to the cytosol and to peroxisomes and is targeted to peroxisomes via association with Mdh3 and a Pex5-dependent piggybacking mechanism. This dual localization of Mdh2 contributes to our understanding of the glyoxylate cycle and provides a new perspective on compartmentalization of cellular metabolism, which is critical for the perception of metabolic disorders and aging. © 2020. Published by The Company of Biologists Ltd.

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