Affordable Access

deepdyve-link
Publisher Website

Picosecond dynamics in haemoglobin from different species: A quasielastic neutron scattering study

Authors
  • Stadler, AM
  • Garvey, CJ
  • Embs, JP
  • Koza, MM
  • Unruh, T
  • Artmann, G
  • Zaccai, G
Publication Date
Oct 01, 2014
Identifiers
DOI: 10.1016/j.bbagen.2014.06.007
OAI: oai:apo-prod.ansto.gov.au:10238/7978
Source
ANSTO Publications Online
Keywords
Language
English
License
Unknown
External links

Abstract

Abstract Background Dynamics in haemoglobin from platypus (Ornithorhynchus anatinus), chicken (Gallus gallus domesticus) and saltwater crocodile (Crocodylus porosus) were measured to investigate response of conformational motions on the picosecond time scale to naturally occurring variations in the amino acid sequence of structurally identical proteins. Methods Protein dynamics was measured using incoherent quasielastic neutron scattering. The quasielastic broadening was interpreted first with a simple single Lorentzian approach and then by using the Kneller–Volino Brownian dynamics model. Results Mean square displacements of conformational motions, diffusion coefficients of internal dynamics and residence times for jump-diffusion between sites and corresponding effective force constants (resilience) and activation energies were determined from the data. Conclusions Modifications of the physicochemical properties caused by mutations of the amino acids were found to have a significant impact on protein dynamics. Activation energies of local side chain dynamics were found to be similar between the different proteins being close to the energy, which is required for the rupture of single hydrogen bond in a protein. General significance The measured dynamic quantities showed significant and systematic variations between the investigated species, suggesting that they are the signature of an evolutionary adaptation process stimulated by the different physiological environments of the respective protein. © 2014,Elsevier B.V.

Report this publication

Statistics

Seen <100 times