The aim of the current study was to detect phytoconstituents present in Guiera Sengalensis extracts using biochemical tests and to evaluate antimicrobial, antioxidant and anti-diabetic potential of the four leaves extracts of the plant using cup-plate diffusion method, DPPH free radical scavenging assay and glucose uptake in yeast cell assay. The plant showed the presence of alkaloid, flavonoid, tannins, sterol, saponin and glycosides. The ethanol extract showed high antimicrobial activity against E.coli with inhibition 30 mm at concentration 100 mg/ml. The plant showed good antioxidant activity with minimum radical scavenging activity 12.3% for petroleum ether extract and maximum activity 74.1% for methanol extract in comparison with standard propyl-gallate 77%. The methanolic extract showed good anti-diabetic activity with minimum increase 25.85 at 40ug/ml and maximum 58.3 at 80ug/ml glucose concentration. The result obtained revealed that this plant is very important from medicinal point of view.