A more rapid increase in night-time temperature compared with day-time temperature and the increased frequency of heat waves associated with climate change present a serious threat to rice (Oryza sativa L.) production and food security. This thesis aims to understand the impact of high night-time temperature (HNT) and high day-time temperature (HDT) on rice grain yield and grain quality and to examine adaptation strategies to cope with high-temperature stresses. Grain yield and quality of a susceptible indica genotype (Gharib) and all tested hybrids, when exposed to HNT in the field, were significantly reduced across seasons, with less average reduction in the dry season than in the wet season, indicating that other environmental factors under field conditions may contribute to impacts of HNT on yield. Among the different yield components, a reduced number of spikelets m−2 significantly contributed to yield loss under HNT followed by the consistently lower single-grain weight across all genotypes, while the impact of the decrease in percentage seed-set was less and season-specific. Lower grain yield and poorer grain quality in susceptible cultivar Gharib were associated with a significant reduction in non-structural carbohydrate translocation after flowering, resulting in reduced grain-filling duration. Increased total nitrogen application did not alleviate the negative impact of HNT. The proposed model approach showed that there were significant differences among cultivars in their changes in source-sink relationships in response to HNT. Given that rice grain yield and quality are challenged by a rise in HDT and HNT, in particular at flowering and during grain filling, differential impacts of HNT and HDT during these critical stages were observed. For the single-grain growth during grain filling, HDT either independently or in combination with HNT exerted greater influences than HNT on the grain filling dynamics, activities of starch metabolism enzymes, temporal starch accumulation patterns, and the process of chalk formation. During flowering, HDT increased spikelet sterility in tested hybrids and hybrids were less tolerant to high temperatures than high-yielding inbred varieties. Moreover, in contrast with HNT, HDT played a dominant role in determining spikelet fertility. Novel observations with a series of snapshots of dynamic fertilization processes demonstrated that disturbances in the pre-fertilization phase were the primary causes for heat-induced spikelet sterility, indicating the effectiveness of employing the early-morning flowering trait for mitigating the impact of heat stress at flowering on rice.