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Physiological relevance and contribution to metal balance of specific and non-specific Metallothionein isoforms in the garden snail, Cantareus aspersus

Authors
  • Höckner, Martina1
  • Stefanon, Karin1
  • de Vaufleury, Annette2
  • Monteiro, Freddy3
  • Pérez-Rafael, Sílvia4
  • Palacios, Òscar4
  • Capdevila, Mercè4
  • Atrian, Sílvia3
  • Dallinger, Reinhard1
  • 1 University of Innsbruck, Institute of Zoology, Innsbruck, 6020, Austria , Innsbruck (Austria)
  • 2 University of Franche-Comté, Department of Chrono-Environment, Besançon Cedex, 25030, France , Besançon Cedex (France)
  • 3 Universitat de Barcelona, Departament de Genètica, Facultat de Biologia, Barcelona, 08028, Spain , Barcelona (Spain)
  • 4 Universitat Autònoma de Barcelona, Departament de Química, Facultat de Ciències, Barcelona, Bellaterra, 08193, Spain , Barcelona (Spain)
Type
Published Article
Journal
BioMetals
Publisher
Springer-Verlag
Publication Date
May 31, 2011
Volume
24
Issue
6
Pages
1079–1092
Identifiers
DOI: 10.1007/s10534-011-9466-x
Source
Springer Nature
Keywords
License
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Abstract

Variable environmental availability of metal ions represents a constant challenge for most organisms, so that during evolution, they have optimised physiological and molecular mechanisms to cope with this particular requirement. Metallothioneins (MTs) are proteins that play a major role in metal homeostasis and as a reservoir. The MT gene/protein systems of terrestrial helicid snails are an invaluable model for the study of metal-binding features and MT isoform-specific functionality of these proteins. In the present study, we characterised three paralogous MT isogenes and their expressed products in the escargot (Cantareus aspersus). The metal-dependent transcriptional activation of the three isogenes was assessed using quantitative Real Time PCR. The metal-binding capacities of the three isoforms were studied by characterising the purified native complexes. All the data were analysed in relation to the trace element status of the animals after metal feeding. Two of the three C.aspersus MT (CaMT) isoforms appeared to be metal-specific, (CaCdMT and CaCuMT, for cadmium and copper respectively). A third isoform (CaCd/CuMT) was non-specific, since it was natively recovered as a mixed Cd/Cu complex. A specific role in Cd detoxification for CaCdMT was revealed, with a 80–90% contribution to the Cd balance in snails exposed to this metal. Conclusive data were also obtained for the CaCuMT isoform, which is involved in Cu homeostasis, sharing about 30–50% of the Cu balance of C. aspersus. No apparent metal-related physiological function was found for the third isoform (CaCd/CuMT), so its contribution to the metal balance of the escargot may be, if at all, of only marginal significance, but may enclose a major interest in evolutionary studies.

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