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Physicochemical characterization of a PEGylated liposomal drug formulation using capillary electrophoresis.

Authors
  • Franzen, Ulrik1
  • Vermehren, Charlotte
  • Jensen, Henrik
  • Østergaard, Jesper
  • 1 Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark. , (Denmark)
Type
Published Article
Journal
Electrophoresis
Publisher
Wiley (John Wiley & Sons)
Publication Date
Mar 01, 2011
Volume
32
Issue
6-7
Pages
738–748
Identifiers
DOI: 10.1002/elps.201000552
PMID: 21365657
Source
Medline
License
Unknown

Abstract

In this work, the applicability of using CE to perform a physicochemical characterization of a PEGylated liposomal drug formulation of the anti-cancer agent oxaliplatin was examined. Characterization of the liposomal drug formulation using CE instrumentation encompassed: determination of the electrophoretic mobilities, size determination by Taylor dispersion analysis and interaction studies. Electrophoretic mobilities determined by CE were compared with the results obtained by laser Doppler electrophoresis, which were found to be subject to larger variation. Average hydrodynamic diameters of the liposome preparations, as determined by Taylor dispersion analysis, were in the range of 61-84 nm and were compared with the results obtained by dynamic light scattering. Interactions between oxaliplatin (and paracetamol) and the PEGylated liposome were non-detectable by CE frontal analysis as well as by liposome electrokinetic chromatography. In contrast, for the more lipophilic compound propranolol, apparent liposome-aqueous phase distribution coefficients (D(lip) ) were successfully determined by both electrokinetic chromatography (log D(lip) =2.10) and by CE frontal analysis (log D(lip) =2.14). It is envisioned that CE and capillary-based techniques, including Taylor dispersion analysis, will be useful tools for the characterization of nanoparticulate (e.g. liposomal) drug formulations.

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