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Physical and genetic map of the Bacteroides fragilis YCH46 chromosome.

Authors
  • Kuwahara, Tomomi
  • Sarker, Mahfuzur Rahman
  • Ugai, Hideyo
  • Akimoto, Shigeru
  • Shaheduzzaman, Syed Mohammed
  • Nakayama, Haruyuki
  • Miki, Tsuyoshi
  • Ohnishi, Yoshinari
Type
Published Article
Journal
FEMS microbiology letters
Publication Date
Feb 05, 2002
Volume
207
Issue
2
Pages
193–197
Identifiers
PMID: 11958939
Source
Medline
License
Unknown

Abstract

The chromosome of Bacteroides fragilis strain YCH46 was shown to be a single circular DNA molecule of about 5.3 Mb having 16 NotI, seven AscI, and six I-CeuI sites. A physical map of the chromosome was constructed by four independent experimental approaches: linking clone analysis, cross-Southern hybridization, partial restriction digestion, and two-dimensional pulsed-field gel electrophoresis. Six rRNA operons and 10 known genes were localized on the physical map.

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