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Photosensitizing mechanism and identification of levofloxacin photoproducts at ambient UV radiation.

Authors
  • Dwivedi, Ashish
  • Mujtaba, Syed F
  • Kushwaha, Hari N
  • Ali, Daoud
  • Yadav, Neera
  • Singh, S K
  • Ray, Ratan S
Type
Published Article
Journal
Photochemistry and Photobiology
Publisher
Wiley (Blackwell Publishing)
Publication Date
2012
Volume
88
Issue
2
Pages
344–355
Identifiers
DOI: 10.1111/j.1751-1097.2011.01068.x
PMID: 22211524
Source
Medline
License
Unknown

Abstract

Levofloxacin (LVFX) is a broad spectrum third generation fluoroquinolone antibiotic, used in the treatment of severe or life-threatening bacterial infections. Photosensitizing mechanism of LVFX was investigated under the ambient environmental intensities of UV-A, UV-B and sunlight exposure. Phototoxic effects of LVFX were assessed on NIH-3T3 and HaCaT cell lines. Results identified first time three photoproducts of LVFX at ambient levels of UV-R by LC-MS/MS. The generation of reactive oxygen species (ROS) was investigated photochemically as well as intracellularly in HaCaT cell line. ROS were significantly quenched by specific quenchers like DABCO, NaN(3), D-mannitol and NAC. Photosensitized LVFX caused lipid peroxidation at different concentrations. Quenching study with superoxide dismutase confirms the LVFX-induced lipid photoperoxidation. Further, photocytotoxicity of LVFX showed significant reduction in cell viability by MTT and neutral red uptake assays. LVFX caused cell arrest in G2/M phases as well as induced apoptosis through ROS-dependent pathway. In addition, photosensitized LVFX also induced upregulation of p21 and Bax/Bcl-2 genes ratio. India is a tropical country and most of the human activities such as agriculture, commerce, sports, etc. take place in bright sunlight; therefore, photosensitive LVFX may lead to skin/ocular disorders and immune suppression. Information is needed regarding the phototoxicity of LVFX for human safety.

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