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A photoelectrochemical enzyme biosensor based on functionalized hematite microcubes for rutin determination by square-wave voltammetry

Authors
  • Mattos, Gabriel J.1
  • Salamanca-Neto, Carlos A. R.1
  • Barbosa, Eduardo C. M.1
  • Camargo, Pedro H. C.2
  • Dekker, Robert F. H.3
  • Barbosa-Dekker, Aneli M.
  • Sartori, Elen R.1
  • 1 Universidade Estadual de Londrina, Centro de Ciências Exatas, Rodovia Celso Garcia Cid, PR 445, Km 380, Cx.P. 10.011, Londrina, PR, CEP: 86057-970, Brazil , Londrina (Brazil)
  • 2 Universidade de São Paulo, Instituto de Química, São Paulo, SP, CEP: 05508-000, Brazil , São Paulo (Brazil)
  • 3 Universidade Tecnológica Federal do Paraná, Câmpus Londrina, Londrina, PR, CEP: 86036-370, Brazil , Londrina (Brazil)
Type
Published Article
Journal
Microchimica Acta
Publisher
Springer-Verlag
Publication Date
Jan 06, 2021
Volume
188
Issue
1
Identifiers
DOI: 10.1007/s00604-020-04659-z
Source
Springer Nature
Keywords
License
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Abstract

A photoelectrochemical biosensing strategy for the highly sensitive detection of the flavonoid rutin was developed by synergizing the photoelectrocatalytic properties of hematite (α-Fe2O3) decorated with palladium nanoparticles (PdNPs) and the biocatalysis towards laccase-based reactions. The integration of α-Fe2O3.PdNPs with a polyphenol oxidase as a biorecognition element yields a novel biosensing platform. Under visible light irradiation, the photoactive biocomposite can generate a stable photocurrent, which was found to be directly dependent upon the concentration of rutin. Under the optimal experimental conditions, the cathodic photocurrent, measured at 0.33 V vs. Ag/AgCl, from the square-wave voltammograms presented a linear dependence on the rutin concentration within the range of 0.008–30.0 × 10−8 mol L−1 (sensitivity: 1.7 μA·(× 10−8 M−1)·cm−2), with an experimental detection limit (S/N = 3) of 8.4 × 10−11 mol L−1. The proposed biosensor device presented good selectivity towards rutin in the presence of various organic compounds and inorganic ions, demonstrating the potential application of this biosensing platform in complex matrices. This bioanalytical device also exhibited excellent operational and analytical properties, such as intra-day (standard deviation, SD = 0.21%) and inter-day (SD = 1.30%) repeatability, and long storage stability (SD = 2.80% over 30 days). Graphical abstract

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