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Photodynamic inactivation of clinical isolates of Candida using Photodithazine®.

Authors
  • Dovigo, L N
  • Carmello, J C
  • Carvalho, M T
  • Mima, E G
  • Vergani, C E
  • Bagnato, V S
  • Pavarina, A C
Type
Published Article
Journal
Biofouling
Publisher
Informa UK (Taylor & Francis)
Publication Date
Jan 01, 2013
Volume
29
Issue
9
Pages
1057–1067
Identifiers
DOI: 10.1080/08927014.2013.827668
PMID: 24025068
Source
Medline
License
Unknown

Abstract

This study evaluated the photodynamic inactivation (PDI) mediated by Photodithazine(®) (PDZ) against 15 clinical isolates of Candida albicans, Candida glabrata and Candida tropicalis. Each isolate, in planktonic and biofilm form, was exposed to PDI by assessing a range of PDZ concentrations and light emitting diode fluences. Cell survival of the planktonic suspensions was determined by colony forming units (CFU ml(-1)). The antifungal effects of PDI against biofilms were evaluated by CFU ml(-1) and metabolic assay. Data were analyzed by non-parametric tests (α = 0.05). Regardless of the species, PDI promoted a significant viability reduction of planktonic yeasts. The highest reduction in cell viability of the biofilms was equivalent to 0.9 log10 (CFU ml(-1)) for C. albicans, while 1.4 and 1.5 log10 reductions were obtained for C. tropicalis and C. glabrata, respectively. PDI reduced the metabolic activity of biofilms by 62.1, 76.0, and 76.9% for C. albicans, C. tropicalis, and C. glabrata, respectively. PDZ-mediated PDI promoted significant reduction in the viability of Candida isolates.

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