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Photobiological properties of methylene violet.

Authors
  • Morrison, H
  • Mohammad, T
  • Kurukulasuriya, R
Type
Published Article
Journal
Photochemistry and Photobiology
Publisher
Wiley (Blackwell Publishing)
Publication Date
Aug 01, 1997
Volume
66
Issue
2
Pages
245–252
Identifiers
PMID: 9277144
Source
Medline
License
Unknown

Abstract

The interaction of methylene violet (MV) and 4-bromomethylene violet (BrMV) with calf thymus and supercoiled phi X174 phage RF I DNA is reported. Measurements employing UV-visible absorption spectroscopy and equilibrium dialysis give evidence for the formation of complexes by each dye with DNA in the dark. They covalently bind to DNA, and MV nicks DNA, when the nucleic acid/dye mixtures are irradiated with visible light in a deoxygenated environment. Quantum efficiencies for singlet oxygen formation are 0.27 and 0.25 for MV and BrMV, respectively. A higher value (0.49) is observed for 4-iodomethylene violet (IMV).

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