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Photoaffinity labeling of a P3 purinoceptor-like protein purified from rat brain membranes.

Authors
  • Saitoh, Y
  • Nakata, H
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Feb 15, 1996
Volume
219
Issue
2
Pages
469–474
Identifiers
PMID: 8605011
Source
Medline
License
Unknown

Abstract

We have found a novel adenosine binding protein in rat brain membranes. Based on the ligand binding specificities, this protein is not classified into any known P1 or P2 purinoceptors but is likely to be classified into the putative P3 purinoceptor which was recently proposed. This P3 purinoceptor-like protein was partially purified from rat brain membranes and characterized further by photoaffinity labeling with 8-azidoadenosine-5'-[gamma-32P]triphosphate. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the labeled preparations revealed that the probe is incorporated specifically into a single band with an approximate molecular size of 54 kDa.

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