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A phosphorimager-based filter binding thyroid hormone receptor competition assay for chemical screening.

Authors
  • Chapo, Joseph
  • Peng, Yanyu
  • Pitts, Kelly R
Type
Published Article
Journal
Journal of pharmacological and toxicological methods
Publication Date
Jan 01, 2007
Volume
56
Issue
1
Pages
28–33
Identifiers
PMID: 17276701
Source
Medline
License
Unknown

Abstract

These data suggest that filtration and phosphorimaging adequately and properly reproduces binding values associated with THR competition. Further, this method gave a 3-fold reduction in time and a 40-fold reduction in radioactive waste over the column-based method. These reductions allow for a substantial increase in assay throughput. Taken together, these data suggest that en masse filtration and phosphorimaging is an efficient and tractable method for verifying large groups of putative T3 competitors in vitro.

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