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Phosphopeptides derived from in vitro phosphorylated E. coli rna polymerase bind to DNA and affect DNA transcription

Authors
  • Cardellini, Elena1
  • Piccinini, Gina2
  • Gianfranceschi, Gian Luigi1
  • 1 Università di Perugia, Dipartimento di Biologia Cellulare e Molecolare, Sezione Fisiologia e Biofsica, Perugia, 06123-I, Italy , Perugia
  • 2 Università di Camerino, Dipartimento di Biologia Cellulare, Molecolare e Animale, Camerino, 62032-I, Italy , Camerino
Type
Published Article
Journal
Molecular and Cellular Biochemistry
Publisher
Springer-Verlag
Publication Date
Jan 01, 1998
Volume
178
Issue
1-2
Pages
393–396
Identifiers
DOI: 10.1023/A:1006811413681
Source
Springer Nature
Keywords
License
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Abstract

E. Coli RNA polymerase was phosphorylated with protein kinase CKII and allowed to bind to pBR322. After digestion of the RNA polymerase-pBR322 complex with proteinase K, the phosphopeptides that remained bound to DNA were extracted and analyzed. These phosphopeptides are able to bind again to DNA and to inhibit transcription.

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