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Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy.

Authors
  • Suarez, Maria Fernanda1
  • Piqueras, Maria Carmen2
  • Correa, Leandro3
  • Esposito, Evangelina3
  • Barros, Maria Fernanda3
  • Bhattacharya, Sanjoy K2
  • Urrets-Zavalia, Julio A3
  • Serra, Horacio M1
  • 1 CIBICI-CONICET, Faculty of Chemical Sciences, Department of Clinical Biochemistry, Universidad Nacional de Cordoba, Cordoba, Argentina. , (Argentina)
  • 2 Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida.
  • 3 Department of Ophthalmology, University Clinic Reina Fabiola, Universidad Catolica de Cordoba, Cordoba, Argentina. , (Argentina)
Type
Published Article
Journal
Journal of Cellular Biochemistry
Publisher
Wiley (John Wiley & Sons)
Publication Date
Nov 01, 2017
Volume
118
Issue
11
Pages
3920–3931
Identifiers
DOI: 10.1002/jcb.26045
PMID: 28401586
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Climatic droplet keratopathy (CDK) is an acquired degenerative disease predominantly affecting males over 40 years old. It results in progressive corneal opacities usually affecting both eyes. CDK is multifactorial and its etiology remains unknown. Our recent findings are consistent with CDK pathology being driven by environmental factors with oxidative stress playing an important role (e.g.,, contributing to lipid peroxidation) rather than climate factors. The changes in corneal lipid composition affected by environmental factors remain understudied. The purpose of this study was to systematically investigate phospholipids profile (phosphatidylcholine [PC] and phosphatidylserine [PS]) in corneas from CDK patients using tandem mass spectrometry. Samples from CDK areas and from non-affected areas were obtained from patients diagnosed with CDK who underwent cataract surgery, were subjected to lipid extraction using a modified Bligh and Dyer method; protein concentrations were determined using the Bradford's method. Lipids were identified and subjected to ratiometric quantification using TSQ Quantum Access Max triple quadrupole mass spectrometer, using appropriate class specific lipid standards. All phospholipid classes showed lower total amounts in affected areas compared to control areas from CDK's corneas. Comparative profiles of two phospholipid classes (PC, PS) between CDK areas and control areas showed several common species between them. We also found a few unique lipids that were absent in CDK areas compared to controls and vice versa. Lower amount of phospholipids in CDK areas compared to control areas could be attributed to the lipid peroxidation in the affected corneal regions as a consequence of increased oxidative stress. J. Cell. Biochem. 118: 3920-3931, 2017. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

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