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Phospholipid composition of packed red blood cells and that of extracellular vesicles show a high resemblance and stability during storage.

Authors
  • Laurén, Eva1
  • Tigistu-Sahle, Feven2
  • Valkonen, Sami3
  • Westberg, Melissa2
  • Valkeajärvi, Anne4
  • Eronen, Juha4
  • Siljander, Pia5
  • Pettilä, Ville6
  • Käkelä, Reijo2
  • Laitinen, Saara4
  • Kerkelä, Erja7
  • 1 Finnish Red Cross Blood Service, Kivihaantie 7, 00310 Helsinki, Finland; Department of Intensive Care Medicine, Department of Anesthesiology, Intensive Care and Pain Medicine, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. , (Finland)
  • 2 University of Helsinki, Department of Biosciences, Division of Physiology and Neuroscience, Helsinki, Finland. , (Finland)
  • 3 Finnish Red Cross Blood Service, Kivihaantie 7, 00310 Helsinki, Finland; University of Helsinki, Department of Biosciences, Division of Biochemistry and Biotechnology, Helsinki, Finland; University of Helsinki, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, Helsinki, Finland. , (Finland)
  • 4 Finnish Red Cross Blood Service, Kivihaantie 7, 00310 Helsinki, Finland. , (Finland)
  • 5 University of Helsinki, Department of Biosciences, Division of Biochemistry and Biotechnology, Helsinki, Finland; University of Helsinki, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, Helsinki, Finland. , (Finland)
  • 6 Department of Intensive Care Medicine, Department of Anesthesiology, Intensive Care and Pain Medicine, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. , (Finland)
  • 7 Finnish Red Cross Blood Service, Kivihaantie 7, 00310 Helsinki, Finland. Electronic address: [email protected] , (Finland)
Type
Published Article
Journal
Biochimica et Biophysica Acta
Publisher
Elsevier
Publication Date
Jan 01, 2018
Volume
1863
Issue
1
Pages
1–8
Identifiers
DOI: 10.1016/j.bbalip.2017.09.012
PMID: 28965917
Source
Medline
Keywords
License
Unknown

Abstract

Red blood cells (RBCs) are stored up to 35-42days at 2-6°C in blood banks. During storage, the RBC membrane is challenged by energy depletion, decreasing pH, altered cation homeostasis, and oxidative stress, leading to several biochemical and morphological changes in RBCs and to shedding of extracellular vesicles (EVs) into the storage medium. These changes are collectively known as RBC storage lesions. EVs accumulate in stored RBC concentrates and are, thus, transfused into patients. The potency of EVs as bioactive effectors is largely acknowledged, and EVs in RBC concentrates are suspected to mediate some adverse effects of transfusion. Several studies have shown accumulation of lipid raft-associated proteins in RBC EVs during storage, whereas a comprehensive phospholipidomic study on RBCs and corresponding EVs during the clinical storage period is lacking. Our mass spectrometric and chromatographic study shows that RBCs maintain their major phospholipid (PL) content well during storage despite abundant vesiculation. The phospholipidomes were largely similar between RBCs and EVs. No accumulation of raft lipids in EVs was seen, suggesting that the primary mechanism of RBC vesiculation during storage might not be raft -based. Nonetheless, a slight tendency of EV PLs for shorter acyl chains was observed.

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