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The phenylic hydroxyl group is essential for the induction of stress response by sodium salicylate

Authors
  • Yamagishi, Nobuyuki
  • Tokunaga, Saki
  • Ishihara, Keiichi
  • Saito, Youhei
  • Hatayama, Takumi
Type
Published Article
Journal
Biochemical and Biophysical Research Communications
Publication Date
Jan 01, 2006
Volume
350
Issue
1
Pages
131–137
Identifiers
DOI: 10.1016/j.bbrc.2006.09.008
Source
Elsevier
Keywords
License
Unknown

Abstract

We have shown that sodium salicylate (SA) activates the heat shock promoter and induces the expression of heat shock proteins (Hsps) with a concomitant increase in the thermotolerance of cells. To identify the functional groups of SA necessary for the induction of Hsps, we evaluated the effect of various derivatives of SA using a mammalian cell line containing a reporter gene downstream of an hsp105 promoter. Among the derivatives, the compounds in which the carboxyl group of SA was substituted activated the hsp105 promoter at 37 °C as SA did, but the compounds in which the hydroxyl group was substituted did not. Thus, the phenylic hydroxyl group but not the carboxyl group of SA seemed to be necessary for a stress-induced response. In addition, the orientation of two functional groups on the benzene ring of SA derivatives was also important for the induction of a response. Among these compounds, salicylalcohol which strongly induced the expression of Hsps suppressed the protein aggregation and apoptosis caused by an expanded polyglutamine tract in a cellular model of polyglutamine disease. These findings may aid in the development of novel effective Hsp-inducers.

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