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Phenotypic analysis of a transgenic Drosophila model of Alzheimer's amyloid-β toxicity.

Authors
  • Sekiya, Michiko1, 2
  • Iijima, Koichi M1, 2
  • 1 Department of Neurogenetics, Center for Development of Advanced Medicine for Dementia, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8511, Japan. , (Japan)
  • 2 Department of Experimental Gerontology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan. , (Japan)
Type
Published Article
Journal
STAR protocols
Publication Date
Jun 18, 2021
Volume
2
Issue
2
Pages
100501–100501
Identifiers
DOI: 10.1016/j.xpro.2021.100501
PMID: 33997815
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

For decades, the fruit fly Drosophila melanogaster has been an efficient genetic model to investigate many aspects of human neurodegenerative diseases. Through genetic and pharmacologic approaches, these studies have revealed the molecular mechanisms underlying disease pathogenesis and provided therapeutic implications. Here, we describe a protocol for assessing Alzheimer's disease-related amyloid-β toxicity in a transgenic fly model through biochemical, histological, and behavioral analyses. We also discuss the advantages and limitations of our protocols. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021). © 2021 The Author(s).

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