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Pharmacological Manipulation of Trk, p75NTR, and NGF Balance Restores Memory Deficit in Global Ischemia/Reperfusion Model in Rats

Authors
  • Choucry, Ali Mohamed1, 2
  • Al-Shorbagy, Muhammad Yusuf1, 3
  • Attia, Ahmed Sherif4
  • El-Abhar, Hanan Salah1
  • 1 Cairo University, Department of Pharmacology and Toxicology, Faculty of Pharmacy, Kasr El Aini st., Cairo, 11562, Egypt , Cairo (Egypt)
  • 2 Toyama University, Department of Biochemistry, Graduate School of Medicine and Pharmaceutical Sciences, 2630 Sugitani, Toyama, 930-0194, Japan , Toyama (Japan)
  • 3 New Giza University, School of Pharmacy, Giza, Egypt , Giza (Egypt)
  • 4 Cairo University, Department of Microbiology and Immunology, Faculty of Pharmacy, Kasr El Aini St., Cairo, 11562, Egypt , Cairo (Egypt)
Type
Published Article
Journal
Journal of Molecular Neuroscience
Publisher
Springer-Verlag
Publication Date
Mar 12, 2019
Volume
68
Issue
1
Pages
78–90
Identifiers
DOI: 10.1007/s12031-019-01284-1
Source
Springer Nature
Keywords
License
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Abstract

Long-term memory impairment is reported in more than 50% of cardiac arrest survivors. Monosialoganglioside (GM1) provided neuroprotection in experimental models of stroke but failed to replicate its promise clinically for unknown reasons. GM1 stimulates the release of nerve growth factor (NGF), which is synthesized as a precursor protein (pro-NGF) that either mediates apoptosis through the p75 neurotrophin receptor (p75NTR) or is cleaved by the protease furin (FUR) to yield mature NGF, the latter supporting survival through tropomyosin kinase receptor (Trk). The flavanol epicatechin (EPI) inhibits p75NTR-mediated signaling and apoptosis by pro-NGF. The aim of the current work is to test whether these two drugs affect, or communicate with, each other in the setting of CNS injuries. Using the two-vessel occlusion model of global ischemia/reperfusion (I/R), we tested if pharmacological modulation of Trk, p75NTR, and NGF balance with GM1, EPI, and their combination, can correct the memory deficit that follows this insult. Finally, we tested if FUR insufficiency and/or p75NTR-mediated apoptosis negatively affect the neurotherapeutic effect of GM1. Key proteins for Trk and p75NTR, FUR, and both forms of NGF were assessed. All treatment regiments successfully improved spatial memory retention and acquisition. A week after the insult, most Trk and p75NTR proteins were normal, but pro/mature NGF ratio remained sharply elevated and was associated with the poorest memory performance. Pharmacological correction of this balance was achieved by reinforcing Trk and p75NTR signaling. GM1 increased FUR levels, while concomitant administration of EPI weakened GM1 effect on pro-survival Trk and p75NTR mediators. GM1 neuroprotection is therefore not limited by FUR but could be dependent on p75NTR. Graphical Abstract"."

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