Affordable Access

deepdyve-link
Publisher Website

PGSE Is a Novel Enhancer Regulating the Proteoglycan Pathway of the Mammalian Golgi Stress Response.

Authors
  • Sasaki, Kanae1
  • Komori, Ryota1
  • Taniguchi, Mai1
  • Shimaoka, Akie1
  • Midori, Sachiko1
  • Yamamoto, Mayu1
  • Okuda, Chiho1
  • Tanaka, Ryuya1
  • Sakamoto, Miyu1
  • Wakabayashi, Sadao1
  • Yoshida, Hiderou1
  • 1 Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo.
Type
Published Article
Journal
Cell Structure and Function
Publisher
Japan Society for Cell Biology
Publication Date
Jan 11, 2019
Volume
44
Issue
1
Pages
1–19
Identifiers
DOI: 10.1247/csf.18031
PMID: 30487368
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The Golgi stress response is a homeostatic mechanism that augments the functional capacity of the Golgi apparatus when Golgi function becomes insufficient (Golgi stress). Three response pathways of the Golgi stress response have been identified in mammalian cells, the TFE3, HSP47 and CREB3 pathways, which augment the capacity of specific Golgi functions such as N-glycosylation, anti-apoptotic activity and pro-apoptotic activity, respectively. On the contrary, glycosylation of proteoglycans (PGs) is another important function of the Golgi, although the response pathway upregulating expression of glycosylation enzymes for PGs in response to Golgi stress remains unknown. Here, we found that expression of glycosylation enzymes for PGs was induced upon insufficiency of PG glycosylation capacity in the Golgi (PG-Golgi stress), and that transcriptional induction of genes encoding glycosylation enzymes for PGs was independent of the known Golgi stress response pathways and ER stress response. Promoter analyses of genes encoding these glycosylation enzymes revealed the novel enhancer elements PGSE-A and PGSE-B (the consensus sequences are CCGGGGCGGGGCG and TTTTACAATTGGTC, respectively), which regulate their transcriptional induction upon PG-Golgi stress. From these observations, the response pathway we discovered is a novel Golgi stress response pathway, which we have named the PG pathway.Key words: Golgi stress, proteoglycan, ER stress, organelle zone, organelle autoregulation.

Report this publication

Statistics

Seen <100 times