Affordable Access

deepdyve-link
Publisher Website

PGDH family genes differentially affect Arabidopsis tolerance to salt stress.

Authors
  • Rosa-Téllez, Sara1
  • Anoman, Armand D1
  • Alcántara-Enguídanos, Andrea1
  • Garza-Aguirre, Raúl Alejandro1
  • Alseekh, Saleh2
  • Ros, Roc3
  • 1 Departament de Biologia Vegetal, Facultat de Farmàcia, Universitat de València, Spain; Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BIOTECMED), Universitat de València. Dr Moliner 50, 46100, Burjassot, Spain. , (Spain)
  • 2 Max Planck Institut für Molekulare Pflanzenphysiologie, 14476, Potsdam-Golm, Germany. , (Germany)
  • 3 Departament de Biologia Vegetal, Facultat de Farmàcia, Universitat de València, Spain; Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BIOTECMED), Universitat de València. Dr Moliner 50, 46100, Burjassot, Spain. Electronic address: [email protected] , (Spain)
Type
Published Article
Journal
Plant science : an international journal of experimental plant biology
Publication Date
Jan 01, 2020
Volume
290
Pages
110284–110284
Identifiers
DOI: 10.1016/j.plantsci.2019.110284
PMID: 31779918
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The first step in the Phosphorylated Pathway of serine (Ser) Biosynthesis (PPSB) is catalyzed by the enzyme Phosphoglycerate Dehydrogenase (PGDH), coded in Arabidopsis thaliana by three genes. Gene expression analysis indicated that PGDH1 and PGDH2 were induced, while PGDH3 was repressed, by salt-stress. Accordingly, PGDH3 overexpressing plants (Oex PGDH3) were more sensitive to salinity than wild type plants (WT), while plants overexpressing PGDH1 (Oex PGDH1) performed better than WT under salinity conditions. Oex PGDH1 lines displayed lower levels of the salt-stress markers proline and raffinose in roots than WT under salt-stress conditions. Besides, the ratio of oxidized glutathione (GSSG) without and with salt-stress was the highest in Oex PGDH1, and the lowest in Oex PGDH3 compared to WT. These results corroborated that PGDH3 activity could be detrimental, while PGDH1 activity could be beneficial for plant salt tolerance. Under salt-stress conditions, PGDH1 overexpression increased Ser content only in roots, while PGDH3 overexpression increased the amino acid level in both aerial parts and roots, compared to the WT. Our results indicate that the response of PGDH family genes to salt-stress depends on the specific gene studied and that increases in Ser content are not always correlated with enhanced plant salt tolerance. Copyright © 2019 Elsevier B.V. All rights reserved.

Report this publication

Statistics

Seen <100 times