Affordable Access

PFRED: A computational platform for siRNA and antisense oligonucleotides design

  • Sciabola, Simone
  • Xi, Hualin
  • Cruz, Dario
  • Cao, Qing
  • Lawrence, Christine
  • Zhang, Tianhong
  • Rotstein, Sergio
  • Hughes, Jason D.
  • Caffrey, Daniel R.
  • Stanton, Robert V.
Publication Date
Jan 22, 2021
[email protected]
External links


PFRED a software application for the design, analysis, and visualization of antisense oligonucleotides and siRNA is described. The software provides an intuitive user-interface for scientists to design a library of siRNA or antisense oligonucleotides that target a specific gene of interest. Moreover, the tool facilitates the incorporation of various design criteria that have been shown to be important for stability and potency. PFRED has been made available as an open-source project so the code can be easily modified to address the future needs of the oligonucleotide research community. A compiled version is available for downloading at as a java Jar file. The source code and the links for downloading the precompiled version can be found at

Report this publication


Seen <100 times