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Perspectives for preclinical mouse models of glaucoma after Boston keratoprosthesis type 1.

Authors
  • Geoffrion, Dominique1
  • Robert, Marie-Claude2
  • Chodosh, James3
  • Di Polo, Adriana4
  • Harissi-Dagher, Mona5
  • 1 Department of Ophthalmology, Centre hospitalier de l'Université de Montréal (CHUM), 1051 Sanguinet, D.01.2273, Montreal, Quebec, H2X 3E4, Canada; Department of Experimental Surgery, Faculty of Medicine, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec, H3G 0B1, Canada. , (Canada)
  • 2 Department of Ophthalmology, Centre hospitalier de l'Université de Montréal (CHUM), 1051 Sanguinet, D.01.2273, Montreal, Quebec, H2X 3E4, Canada. , (Canada)
  • 3 Department of Ophthalmology, Massachusetts Eye and Ear, Harvard Medical School, 243 Charles Street, Boston, MA, 02114 - 3002, United States. , (United States)
  • 4 Department of Ophthalmology, Centre hospitalier de l'Université de Montréal (CHUM), 1051 Sanguinet, D.01.2273, Montreal, Quebec, H2X 3E4, Canada; Department of Neurosciences, Université de Montréal, Centre de recherche du Centre hospitalier de l'Université de Montréal (CRCHUM), 900 Saint-Denis, R09.720, Montreal, Quebec, H2X 0A9, Canada. , (Canada)
  • 5 Department of Ophthalmology, Centre hospitalier de l'Université de Montréal (CHUM), 1051 Sanguinet, D.01.2273, Montreal, Quebec, H2X 3E4, Canada. Electronic address: [email protected] , (Canada)
Type
Published Article
Journal
Experimental Eye Research
Publisher
Elsevier
Publication Date
Jul 01, 2021
Volume
208
Pages
108615–108615
Identifiers
DOI: 10.1016/j.exer.2021.108615
PMID: 33971222
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Animal models of the Boston keratoprosthesis type 1 (KPro) are needed to study glaucoma damage after KPro implantation to control for confounding comorbidities common in human KPro recipients. The purpose of this study was to determine the feasibility of establishing a reproducible mouse model of glaucoma after KPro surgery, specifically that of a miniaturized mouse model of KPro (mKPro). In the present study, a total of 20 corneas of donor C57BL/6 mice (n = 10) were implanted in one eye of each recipient BALB/C mouse (n = 20), assembled as part of the mKPro, either with or without intraoperative lensectomy. Main feasibility outcomes consisted in incidence rates of loss of tone, capsule nicking, and lens extrusion, as well as acquisition of posterior segment optical coherence tomography (OCT) images. With lensectomy (n = 10), loss of ocular tone and retinal detachment occurred in 100% of mice. Without lensectomy (n = 10), capsule nicking and opening, as well as lens extrusion, occurred in 80% of mice. Causes of these complications included the large proportion of intraocular volume occupied by the lens, the shallow anterior chamber, and thus the lack of available intraocular volume to implant the KPro if the lens remains present. Successful mouse KPro surgery may require a great deal of practice to be useful as a reproducible model. Animal KPro models ought to be pursued further by research teams in future studies. Copyright © 2021 Elsevier Ltd. All rights reserved.

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