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Perspectives from Ten Years of Protist Studies by High-Throughput Metabarcoding.

Authors
  • Santoferrara, Luciana1, 2
  • Burki, Fabien3
  • Filker, Sabine4
  • Logares, Ramiro5
  • Dunthorn, Micah6
  • McManus, George B2
  • 1 Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT, USA.
  • 2 Department of Marine Sciences, University of Connecticut, Groton, CT, USA.
  • 3 Department of Organismal Biology, Program in Systematic Biology, and Science for Life Laboratory, Uppsala University, Uppsala, Sweden. , (Sweden)
  • 4 Department of Molecular Ecology, University of Kaiserslautern, Kaiserslautern, Germany. , (Germany)
  • 5 Institute of Marine Sciences (ICM), CSIC, Barcelona, Spain. , (Spain)
  • 6 Department of Eukaryotic Microbiology, University of Duisburg-Essen, Essen, Germany. , (Germany)
Type
Published Article
Journal
The Journal of eukaryotic microbiology
Publication Date
Sep 01, 2020
Volume
67
Issue
5
Pages
612–622
Identifiers
DOI: 10.1111/jeu.12813
PMID: 32498124
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

During the last decade, high-throughput metabarcoding became routine for analyzing protistan diversity and distributions in nature. Amid a multitude of exciting findings, scientists have also identified and addressed technical and biological limitations, although problems still exist for inference of meaningful taxonomic and ecological knowledge based on short DNA sequences. Given the extensive use of this approach, it is critical to settle our understanding on its strengths and weaknesses and to synthesize up-to-date methodological and conceptual trends. This article summarizes key scientific and technical findings, and identifies current and future directions in protist research that uses metabarcoding. © 2020 International Society of Protistologists.

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