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A personal computer program using a 4-component logit for the evaluation of ELISA calibration curves with unknown asymptotic values.

Authors
  • Winkler, G
  • Heinz, F X
  • Kunz, C
Type
Published Article
Journal
Computer Methods and Programs in Biomedicine
Publisher
Elsevier
Publication Date
Apr 01, 1986
Volume
22
Issue
2
Pages
167–170
Identifiers
PMID: 3635455
Source
Medline
License
Unknown

Abstract

A BASIC program is described for the calculation of ELISA calibration curves using a 4-component logit for the linearisation of the sigmoidal shape. The difficulty of estimating the four logit parameters without knowledge of upper and lower asymptotic values is overcome by statistical analysis of the experimental data. The program is run on an IBM-PC and the curves are plotted by a HP7475A plotter.

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