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Persistent contamination of <i>Salmonella</i>, <i>Campylobacter</i>, <i>Escherichia coli </i>and <i>Staphylococcus aureus </i>at a Broiler Farm in New Zealand.

Authors
  • Castaneda-Gulla, Kristine Mejia1
  • Mutukumira, Anthony N2
  • Sattlegger, Evelyn3
  • 1 Massey University College of Sciences, 117655, School of Natural and Mathematical Sciences, Auckland, New Zealand ; [email protected] , (New Zealand)
  • 2 Massey University - Albany Campus, 168219, Massey Institute of Food Science and Technology , Private Bag 102904 , Auckland , North Shore City, New Zealand , 0745 ; [email protected] , (New Zealand)
  • 3 Massey University College of Sciences, 117655, School of Natural and Mathematical Sciences, Auckland, New Zealand ; [email protected] , (New Zealand)
Type
Published Article
Journal
Canadian Journal of Microbiology
Publisher
Canadian Science Publishing
Publication Date
Nov 13, 2019
Identifiers
DOI: 10.1139/cjm-2019-0280
PMID: 31721603
Source
Medline
Language
English
License
Unknown

Abstract

Intensive poultry production due to public demand raises the risk of contamination, creating potential foodborne hazards to consumers. The prevalence and microbial load of pathogens <i>Campylobacter</i>, <i>Salmonella</i>, <i>S. aureus</i> and <i>E. coli </i>was determined by standard methods at the farm level. After disinfection, swab samples collected on wall crevices, drinkers and vents were heavily contaminated, as accumulated organic matter and dusts were likely protecting pathogens against the disinfectants used. The annex floor also showed high microbial concentrations, suggesting the introduction of pathogens from the external environments, highlighting the importance of erecting hygiene barriers at the entrance of the main shed. Therefore, pathogen control measures and proper application of disinfectants are recommended as intervention strategies. Additionally, qPCR was evaluated as a quantification tool. qPCR showed limitations with samples containing low microbial counts due to the low detection limit of the method. Thus, bacterial pre-enrichment of test samples may be necessary to improve the detection of pathogens by qPCR.

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